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To test this hypothesis, we will synthesize a small library of enzyme-directed imidazoquinolines with cages for beta-galactosidase, alpha-mannosidase, and nitroreductase, and compare their conversion by exogenous enzymes ex vivo and native enzymes upregulated in cancer cells in vitro.

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The good news is that leprosy is curable, and the World Health Organization offers free treatment to all diagnosed cases. The bad news is that we still do not know how leprosy is transmitted from one person to another. We hypothesize that an unappreciated mode of leprosy infection is breastfeeding, and that milk produced by infected women can contain Mycobacterium leprae, the bacterium that causes the disease.

Using molecular techniques that I helped develop, I am investigating this hypothesis in collaboration with clinicians and researchers at Anandaban Hospital outside of Kathmandu, Nepal. The study is ongoing, but the results will likely impact recommendations for treatment of breastfeeding women and their infants in leprosy-endemic regions around the globe.

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The corresponding theory, quantum mechanics, describes a mysterious and complex world, riddled with concepts and phenomena that are not well understood. With the general trend of technology becoming smaller, it is crucial to understand the physics encountered on the nanoscale. By cooling down large collections of atoms to temperatures near absolute zero, we are able to study macroscopic quantum systems with extreme control and tunability. Ultracold atoms ultimately bring the quantum world to our fingertips.

However, our understanding of this process remains far from complete. Filling the gaps in knowledge regarding this fundamental process will benefit humankind in a myriad of ways ranging from securing food production to creating artificial photosynthetic devices to harness light energy. Joseph Hantho A Comparison of Enzyme-Directed Pro-Immunostimulants for Cancer Immunotherapy Herein, we propose the synthesis and activity of enzyme-directed pro-immunostimulants to determine their efficacy cancer immunotherapy.

Immunostimulants have long shown efficacious anticancer effects, but intravenous administration yields systemic toxicity.

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Previously, substrate cages for beta-galactosidase, alpha-mannosidase, and nitroreductase enzymes have been employed for enzyme-directed targeting approaches. However, the bystander effect, the phenomenon when a prodrug is activated by one cell and interacts with or kills another, has shown off-target toxicity in surrounding tissues caused by diffusion of the drug away from tumor cells. By using a caged immunostimulant, diffusion of the active immunostimulant would be desirable, as immune cells are commonly found in or surrounding tumor microenvironements.

Modification of the critical moiety of a known immunostimulant, either through oxidation or by covalent attachment of an enzyme-cleavable cage, will selectively activate anticancer immune responses only in the presence of the corresponding enzyme.

To test this hypothesis, we will synthesize a small library of enzyme-directed imidazoquinolines with cages for beta-galactosidase, alpha-mannosidase, and nitroreductase, and compare their conversion by exogenous enzymes ex vivo and native enzymes upregulated in cancer cells in vitro.

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The good news is that leprosy is curable, and the World Health Organization offers free treatment to all diagnosed cases. The bad news is that we still do not know how leprosy is transmitted from one person to another.

We hypothesize that an unappreciated mode of leprosy infection is breastfeeding, and that milk produced by infected women can contain Mycobacterium leprae, the bacterium that causes the disease. Using molecular techniques that I helped develop, I am investigating this hypothesis in collaboration with clinicians and researchers at Anandaban Hospital outside of Kathmandu, Nepal.

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